Chapter 1 Fluctuation Analysis of Apical Sodium Transport

摘要: Publisher Summary This chapter discusses the fluctuation analysis of apical sodium transport. One biggest problems in studying sodium-selective channel is that its kinetics are very slow and many artifacts arising from chamber design membrane mounting give rise to large-amplitude, low-frequency noise. Such noise can obscure fluctuations due channels. Fluctuation a kinetic tool offers possibility obtaining new information not available using traditional methods. The relationship between current power steady state makes it likely arise blocking-unblocking action conducting channel. possible estimate single-channel density. Although estimates for these quantities obtained an exogenously applied blocking agent, their values appear be relatively independent blocker reflect intrinsic properties. magnitude single probable transport mechanism carrier. Access such study mode regulatory agents including hormones, which stimulate whether actions through increase density or current. Thus far, seems both ADH aldosterone act by increasing rather than Additional parameters measured association dissociation rate constants agent. concludes this technique will become another useful all epitheliologists.