Molecular epidemiological study of nosocomial Enterobacter aerogenes isolates in a Belgian hospital

作者: Sheikh Jalaluddin , Jeanne-Marie Devaster , Robert Scheen , Michele Gerard , Jean-Paul Butzler

DOI: 10.1128/JCM.36.7.1846-1852.1998

关键词:

摘要: In 1995, the rate of isolation Enterobacter aerogenes in Saint-Pierre University Hospital Brussels, Belgium, was higher than that preceding years. A total 45 nosocomial E. strains were collected from 33 patients different units during year, and they isolated 19 respiratory specimens, 13 pus 7 blood 4 urinary 1 catheter specimen, heparin vial. The analyzed to determine their epidemiological relatedness characterized by antibiotic resistance pattern determination, plasmid profiling, genomic fingerprinting macrorestriction analysis with pulsed-field gel electrophoresis (PFGE). majority (82%) multiply resistant commonly used antibiotics. Two major profiles found: most (64%) harbored two plasmids sizes, whereas others (20%) contained a single plasmid. PFGE SpeI and/or XbaI restriction enzymes revealed clone (80%) responsible for causing infections or colonizations throughout this result concordant those obtained slight variations. By comparing results these three methods, profiling found be techniques best suited investigating strains, are therefore proposed as useful tools investigation outbreaks caused organism.

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