Studies on the preparation of rat liver plasma membrane fractions and on their polypeptide patterns

摘要: Plasma membrane and bile canalicular fractions were prepared from rat liver using NaHCO3, NaHCO3–CaCl2, K2HPO4–KH2PO4 buffers (all at pH 7.4). The amount (expressed as milligrams protein per gram liver) of plasma fraction exceeded the each these three media; use NaHCO3–CaCl2 afforded a substantially higher yield both types membranes. two exhibited complex patterns polypeptides (> 30) on sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis. Several reproducible differences in polypeptide observable between fractions; particular, components possibly corresponding to heavy chain myosin actin prominent fraction. effects incubation above Tris–HCl (pH 7.4) studied. Many released typ...