Pre-symptomatic transcriptome changes during cold storage of chilling sensitive and resistant peach cultivars to elucidate chilling injury mechanisms.

摘要: Cold storage induces chilling injury (CI) disorders in peach fruit (woolliness/mealiness, flesh browning and reddening/bleeding) manifested when ripened at shelf life. To gain insight into the mechanisms underlying CI, we analyzed transcriptome of ‘Oded’ (high tolerant) ‘Hermoza’ (relatively tolerant to woolliness, but sensitive bleeding) cultivars pre-symptomatic stages. The expression profiles were compared validated with two previously pools low woolliness) from Pop-DG population. four types cover a wide range sensitivity CI. also investigated ROSMETER that provides information on specificity transcriptomic response oxidative stress. We identified quantitative differences subset core cold responsive genes correlated or tolerance CI harvest during storage, subsets correlating specifically high woolliness browning. Functional analysis indicated elevated levels, related antioxidant systems biosynthesis metabolites activity correlates tolerance. Consistent these results, revealed stress progeny pools, not resistant ‘Oded’. By contrast, induced, dependant manner, gene program involving secondary cell wall pectins. Furthermore, our results while ethylene is tolerance, differential auxin subcellular accumulation signaling may play role determining sensitivity/tolerance. In addition, sugar partitioning demand tolerance/sensitive mechanism. indicates vesicle trafficking, membrane dynamics cytoskeleton organization could have case browning, suggest acetaldehyde together responses increase Our data activated regulated by distribution hormones even before are stored.