FLT3/FLK2 ligand promotes the growth of murine stem cells and the expansion of colony-forming cells and spleen colony-forming units

摘要: The effect of FLT3/FLK2 ligand (FL) on the growth primitive hematopoietic cells was investigated using ThyloSca1+ stem cells. FL observed to interact with a variety factors initiate colony formation by When were stimulated in liquid culture plus interleukin (IL)-3, IL-6, granulocyte colony-stimulating factor (G-CSF), or cell (SCF), capable forming colonies secondary methylcellulose cultures (CFU-c) produced high numbers. However, only IL-6 supported an increase number spleens irradiated mice (CFU-s). Experiments accessory cell-depleted bone marrow (Lin-BM) showed that alone lacks significant activity for progenitor Nevertheless, enhanced granulocyte-macrophage progenitors (CFU-GM) containing SCF, G-CSF, IL-11. In these assays, increased CFU-GM initiating (recruitment), as well per (synergy). Many macroscopic and contained greater than 2 x 10(4) granulocytes macrophages. Therefore, appears function potent costimulus proliferative potential (HPP). also costimulate expansion Lin-BM. contrast, had no growth-promoting affects committed erythrocyte, megakaryocyte, eosinophil, mast lineages.