Beneficial effect of ursodeoxycholic acid on alterations induced by cholestasis of pregnancy in bile acid transport across the human placenta

摘要: Abstract Background/Aims: The existence of impairment in bile acid transport across the placenta during intrahepatic cholestasis pregnancy and effect ursodeoxycholic treatment (1 g/day) were investigated. Methods: Kinetic parameters calculated from experiments carried out on membrane vesicles obtained basal (TPMb, fetal-facing) apical (TPMa, maternal-facing) trophoblast plasma membranes. Bile uptake was measured using varying concentrations [ 14 C]-glycocholate a rapid filtration technique. Results: maximal velocity (Vmax), apparent affinity constant (Kt) efficiency (Ef) (Vmax/Kt) anion:bile exchanger located at TPMb reduced pregnancy. Ursodeoxycholic induced reversal Vmax, Kt Ef to normal values. Owing 3-fold increase with no change Kt, an enhancement ATP-independent TPMa. Both Vmax restored values by acid. Finally, ATP-dependent TPMa, reduction due together more pronounced found. This also reversed Conclusions: These results suggest that systems are impaired Moreover, confirmed beneficial for patients, such as relief pruritus improvement biochemical markers cholestasis, restores ability carry vectorial transfer.