Mutation of cytotoxin-associated gene A affects expressions of antioxidant proteins of Helicobacter pylori

摘要: AIM: To determine if disruption of the cagA gene Helicobacter pylori (H pylori) has an effect on expression other proteins at proteome level. METHODS: Construction a knock out mutant Hp27_ΔcagA (cagA-) via homologous recombination with wild-type strain Hp27 (cagA+) as recipient was performed. The method sonication-urea-CHAPS-DTT employed to extract bacterial from both strains. Soluble were analyzed by two-dimensional electrophoresis (2-DE). Images 2-DE gels digitalized and analyzed. Only spots that had statistical significance in differential selected matrix-assisted laser desorption/ionization-time flight mass spectrometry (MALDI-TOF-MS). Biological information used search protein database identify biological function proteins. RESULTS: expressions between isogenic knocked-out compared. Five high abundance bacteria strains, down-regulated or absently expressed mutants, identified From quantitative point view, are related important antioxidant H pylori, including alkyl hydroperoxide reductase (Ahp), superoxide dismutase (SOD) modulator drug activity (Mda66), respectively, suggesting is maintain normal antioxidative stress ensure persistent colonization host. CONCLUSION: relevant which may be novel mechanism involved pathogenesis.