In vivo and in vitro inhibition of rat liver glutathione transferases activity by extracts from Combretum zeyheri (Combretaceae) and Parinari curatellifolia (Chrysobalanaceae).

摘要: Parinari curatellifolia and Combretum zeyheri are medicinal plants used in Zimbabwe other Southern African countries for stomach ailments, fever, body aches, wound healing, cancer tuberculosis. Glutathione transferases (GSTs) mammalian enzymes that play a significant role the detoxification metabolism of many xenobiotic endogenous compounds as such can interact with exogenous including herbal medicines. The effects leaf extracts on glutathione male Sprague–Dawley rats were investigated vivo vitro after oral administration either ethanol or water each plant. For curatellifolia, 18 Sprague-Dawley administered 0, 500 1000 mg/kg weight corn oil saline. Animals sacrificed 96 h kidney liver samples removed to prepare cytosolic fractions. GST activity was determined using 1-chloro-2, 4-dinitrobezene. zeyheri, twenty four randomly divided into two groups. These groups further three animals each. They given aqueous extract at doses C. 50 mg/kg 200 mg/kg weight. orally by gavage consecutive days cervical dislocation fifth day. In significantly increased kidneys livers whilst ethanolic decreased enzyme both organs. Both inhibited being more potent inhibitor than ethacrynic acid, standard inhibitor. versus inhibition suggests metabolites may be responsible observed vivo. P. dose-dependent decrease but no changes extract. There concentration-dependent GSTs when vitro. exhibited GST-inhibitory an IC50 value 12 μg/mL found 10 μg/mL. This showed this had inductive effect solution Induction would cytoprotective against toxic electrophilic chemicals. Since synthesis prostaglandins, these maybe one reasons makes important use treatment pain fever ethnopharmacology.