Is the formation of cationic lipid-DNA complexes a thermodynamically driven phenomenon? Structure and phase behavior of DC-Chol/DNA complexes say not
作者： Giulio Caracciolo , Daniela Pozzi , Ruggero Caminiti
DOI: 10.1063/1.2243869
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摘要: The currently accepted mechanism of formation cationic lipid-DNA complexes (lipoplexes) relies on the basic assumption that equilibrium structure lipoplexes is regulated by thermodynamics. main consequence neutral are one phase whereas positively (or negatively) charged ones coexist with excess lipid DNA). authors report a small angle x-ray diffraction study made 3β-[N-(N,N-dimethylaminoethane)-carbamoyl]cholesterol and calf thymus Na-DNA. Here show can unbound DNA they claim steric size effects definitely important to determine lipoplexes.
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