Domestication of wild Saccharomyces cerevisiae is accompanied by changes in gene expression and colony morphology

摘要: Although colonies from Saccharomyces cerevisiae laboratory strains are smooth, those isolated nature exhibit a structured fluffy pattern. Environmental scanning electron microscopy shows that the cells within wild connected by extracellular matrix (ECM) material. This material contains protein of about 200 kDa unrelated to flocculins, proteins involved in cell-cell adhesion liquid media. The binds concanavalin A. Within few passages on rich agar medium, switch smooth colony morphology. domestication is accompanied loss ECM and extensive changes gene expression as detected DNA microarrays. 320 genes was changed colonies. major comprise carbohydrate metabolism, cell wall, water channels, Ty-transposons subtelomeric genes, iron homeostasis, vitamin metabolism cycle polarity. growth may represent metabolic strategy for survival yeast under unfavourable conditions switched off felicitous conditions.