Purification and reconstitution of the ryanodine- and caffeine-sensitive Ca2+ release channel complex from muscle sarcoplasmic reticulum.

摘要: Studies in our laboratory are directed towards obtaining a better understanding of the process excitation-contraction coupling muscle, primarily using purified membrane and protein preparations. In excitable tissues, release Ca2+ ions can be triggered by change surface potential (Ebashi, 1976), or it occur via chain voltage-independent steps that involve agonist-induced formation inositol 1,4,5-trisphosphate (IP3) its subsequent binding activation an intracellular receptor/channel complex, IP3 receptor (Van Breemen Saida, 1989). The voltage-dependent mechanism, commonly referred to as excitation -contraction (E-C) coupling, has been most extensively studied skeletal cardiac muscle. striated rapid from compartment, sarcoplasmic reticulum (SR), is action thought communicated SR at specialized areas where comes close contact with tubular infoldings (T-tubule). Spanning gap between two systems bridges (Peachey Armstrong, 1983) which have variously termed “feet” (Franzini-Armstrong, 1970), “bridges” (Somlyo, 1979), “pillars” (Eisenberg Eisenberg, 1982), “spanning” proteins (Caswell Brandt, 1989), now believed identical ryanodine channel complex (Fleischer Inui, 1989; Lai Meissner,