GFAP and nuclear lamins share an epitope recognized by monoclonal antibody J1-31

摘要: Abstract Monoclonal antibody J1-31 was raised against plaque materials taken from brains of patients who had suffered multiple sclerosis (MS). Preliminary characterization the antigen revealed it to be a protein M w 68–70 kDa with both cytoplasmic and nuclear localization. Here we report results isolation peptide sequencing human brains, immunocytochemical analysis in F98 glioma cells. Purification indicate that recognizes form glial fibrillary acidic protein, possibly phosphorylated variant. However, confocal immunocytochemistry western cells raise possibility also epitope found lamins. Analysis expression respect time culture, cell density, DNA synthesis showed developmental relationship: were engaged rapid growth exhibited strong staining nuclei, whereas quiescent did not. We conclude mAB remains useful for studying sclerosis, is likely prove studies dynamics lamins, particularly models wound-healing.