Profiling of primary peripheral blood- and monocyte-derived dendritic cells using monoclonal antibodies from the HLDA10 Workshop in Wollongong, Australia.

摘要: Dendritic cells (DCs) arise from hematopoietic stem and develop into a discrete cellular lineage distinct other leucocytes. Mainly three phenotypically functionally DC subsets are described in the human peripheral blood (PB): plasmacytoid DCs (pDCs), which express key marker CD303 (BDCA-2), two myeloid (CD1c(+) (mDC1) CD141(+) (mDC2)), markers CD1c (BDCA-1) CD141 (BDCA-3), respectively. In addition to these primary cell subsets, can also be generated vitro either CD34(+) stem/progenitor presence of Flt3 (Fms-related tyrosine kinase 3) ligand or CD14(+) monocytes (monocyte-derived (mo-DCs)) granulocyte-macrophage colony-stimulating factor+interleukin-4 (GM-CSF+IL-4). Here we compare reactivity patterns HLDA10 antibodies (monoclonal antibody (mAb)) with pDCs, CD1c(+) DCs, as well CD14(+)-derived mo-DCs cultured for 7 days 100 ng/ml GM-CSF plus 20 ng/ml IL-4. A detailed profiling based on immunophenotyping multicolour flow cytometry analysis is presented. Using panel Workshop mAb, could verify known targets selectively expressed including CD370 selective CD366 both subsets. addition, vimentin heterogeneously all suggesting existence so far not identified