Methodological basis for the optimization of a marine sea-urchin embryo test (SET) for the ecological assessment of coastal water quality.

摘要: The sea-urchin embryo test (SET) has been frequently used as a rapid, sensitive, and cost-effective biological tool for marine monitoring worldwide, but the selection of objective, automatically readable endpoint, stricter quality control to guarantee optimum handling material, identification confounding factors that interfere with response have hampered its widespread routine use. Size increase in minimum n=30 individuals per replicate, either normal larvae or earlier developmental stages, was preferred observer-dependent, discontinuous responses endpoint. Control size after 48 h incubation at 20 degrees C must meet an acceptability criterion 218 microm. In order avoid false positives minimums 32 thousand salinity, 7 pH 2mg/L oxygen, maximum 40 microg/L NH(3) (NOEC) are required media. For situ testing rates be corrected on degree-day basis using 12 threshold.