Dual Ultrastructural Localization of μ-Opioid Receptors and NMDA-Type Glutamate Receptors in the Shell of the Rat Nucleus Accumbens
作者： K. Noelle Gracy , Adena L. Svingos , Virginia M. Pickel
DOI: 10.1523/JNEUROSCI.17-12-04839.1997
关键词:
摘要: The effectiveness of NMDA antagonists in modulating the motor and motivational effects opiates is attributed, part, to functional associations involving receptors μ-opioid (MORs) shell nucleus accumbens (Acb). To determine subcellular sites for potential interactions between opiate ligands this region, we examined ultrastructural localization antipeptide antisera against MOR R1 subunit receptor Acb adult rat brain. MOR-like immunoreactivity (MOR-LI) was seen primarily dendrites, whereas NMDAR1-like (NMDAR1-LI) detected more often axon terminals forming asymmetric synapses. In these profiles, labeling localized mainly extrasynaptic plasma membranes, NMDAR1-LI associated with both synaptic sites. Of 307 MOR-labeled processes, 17.9% dendrites 9.4% also contained NMDAR1-LI. addition, 24.7% containing only MOR-LI were apposed NMDAR1-labeled axons or terminals. We conclude that Acb, output single neurons can be dually modulated by (1) activation same (2) combined presynaptic afferents contacting MOR. colocalization NMDAR1 certain suggests their dual involvement release neurotransmitters region.
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