Rapid resolution by reversed-phase high-performance liquid chromatography of the thylakoid membrane proteins of the photosystem II light-harvesting complex
作者： Lello Zolla , Maria Bianchetti , Anna Maria Timperio , Danilo Corradini
DOI: 10.1016/S0021-9673(97)00447-0
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摘要: This paper presents the results of a study performed to develop rapid and straightforward HPLC method resolve identify protein components photosystem II light-harvesting complex (LHC II), which is major protein-chlorophyll a/b in higher plants. The employs reversed-phase Vydac Protein C4 column containing 5-μm porous butyl silica eluted by 13.5-min linear water-acetonitrile gradient presence 0.1% (v/v) trifluoroacetic acid. Peak identification was denaturing SDS-PAGE analysis fractions eluate collected throughout chromatographic run. procedure allowed Lhcb2, Lhcb1 Lhcb3 LHC II, as well-resolved peaks with highly repeatable retention times, samples isolated from spinach leaves both same different preparations. has proven be suitable for detecting dissimilarities
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sci-hub.se 参考文章(17)
Chlorophyll-proteins of the photosystem II antenna system. Journal of Biological Chemistry. ,vol. 262, pp. 13333- 13341 ,(1987) , 10.1016/S0021-9258(18)45205-2
Parag R. Chitnis, J. Philip Thornber, The major light-harvesting complex of Photosystem II: aspects of its molecular and cell biology. Photosynthesis Research. ,vol. 16, pp. 41- 63 ,(1988) , 10.1007/BF00039485
Ulla K Larsson, Cecilia Sundby, Bertil Andersson, None, Characterization of two different subpopulations of spinach light-harvesting chlorophyll ab-protein complex (LHC II): Polypeptide composition, phosphorylation pattern and association with Photosystem II Biochimica et Biophysica Acta. ,vol. 894, pp. 59- 68 ,(1987) , 10.1016/0005-2728(87)90212-X
Harald Paulsen, CHLOROPHYLL a/b‐BINDING PROTEINS Photochemistry and Photobiology. ,vol. 62, pp. 367- 382 ,(1995) , 10.1111/J.1751-1097.1995.TB02357.X
Beverley R. Green, Dingren Shen, Ruedi Aebersold, Eran Pichersky, Identification of the polypeptides of the major light-harvesting complex of photosystem II (LHCII) with their genes in tomato. FEBS Letters. ,vol. 305, pp. 18- 22 ,(1992) , 10.1016/0014-5793(92)80646-X
O. Machold, The Structure of Light-Harvesting Complex II as Deduced from its Polypeptide Composition and Stoichiometry I: Studies with Vicia faba Journal of Plant Physiology. ,vol. 138, pp. 678- 684 ,(1991) , 10.1016/S0176-1617(11)81315-5
Roberto BASSI, Paola DAINESE, A supramolecular light‐harvesting complex from chloroplast photosystem‐II membranes FEBS Journal. ,vol. 204, pp. 317- 326 ,(1992) , 10.1111/J.1432-1033.1992.TB16640.X
Werner K¨hlbrandt, Da Neng Wang, Three-dimensional structure of plant light-harvesting complex determined by electron crystallography Nature. ,vol. 350, pp. 130- 134 ,(1991) , 10.1038/350130A0
Beverley R. Green, The chlorophyll-protein complexes of higher plant photosynthetic membranes or just what green band is that? Photosynthesis Research. ,vol. 15, pp. 3- 32 ,(1988) , 10.1007/BF00054985
R.J. Porra, W.A. Thompson, P.E. Kriedemann, Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy Biochimica et Biophysica Acta (BBA) - Bioenergetics. ,vol. 975, pp. 384- 394 ,(1989) , 10.1016/S0005-2728(89)80347-0