Targeting the heart with gene therapy-optimized gene delivery methods.

摘要: With evolving knowledge in molecular and cellular cardiology, cardiac gene therapy has already been investigated clinical studies. Different vector systems for have developed recent years. While non-viral vectors, such as plasmid DNA, allow remarkable organ specificity, they are often limited by low transfection efficiency transient expression. In contrast, adenoviral or adeno-associated virus-based vectors transfer the transgene more efficiently, but specificity may be reduced immunogenic properties can limit their applicability. Using advanced transcriptional transductional targeting strategies, viral improved last few Recently, efficient serotypes of viruses identified that show increased transduction rates, thus reducing necessity high virus titers. Combination with specific application techniques, intramyocardial injection, catheter-based perfusion, ultrasound targeted microbubble destruction, retroinfusion further enhance efficiency. This review article will give a broad overview different delivery strategies applied experimental studies heart.