Determination of the minimum number of microsatellite markers for individual genotyping in wild boar (Sus scrofa) using a test with close relatives

摘要: In the context of developing a noninvasive, practicable method for population size estimation in wild boar, we present stepwise procedure to reduce number required microsatellite markers individual genotyping. Step1: an initial marker set 12 loci was tested species specificity with nontarget DNA and resulted exclusion two markers. Step 2: variability test regarding heterozygosity deviations from Hardy–Weinberg equilibrium led rejection further 3: remaining eight were transferability across populations three separate boar sample sets. 4: on basis probability identity values, reduction five possible. 5: novel using tissue samples female boars their embryos provided evidence that four variable one sex are sufficient identification close relatives. 6: feces finally used estimate PCR (PS) genotyping success (GS). conclusion, recommend specific four-marker combination both PS GS >50% reliable noninvasive boar.