Study of antimicrobial effects of several antibiotics and iron oxide nanoparticles on biofilm producing pseudomonas aeruginosa

摘要: Objective(s): Pseudomonas aeruginosa is a nosocomial pathogen resistant to most antimicrobial treatments. Furthermore, it persists in adverse environments thereby forming biofilms on various surfaces. Researchers have therefore focused antibiofilm strategies using nanoparticles due their unique physicochemical properties. Superparamagnetic iron oxide (SIONPs) recently shown possess and anti-biofilm characteristics. In this study, the effects of SIONPs some antibiotics were tested against strong biofilmproducing P. isolates.Materials Methods: 60 isolates P.aeruginosa screened for biofilm formation microtiter plates 0.1%w/v crystal violet (CV) staining. Twenty producing selected further study agents. Microdilution method was used assay twenty susceptible antibiotics. The determined by microdilution 0.1% CV checkerboard dilution technique determine combined imipenem.  Results: isolates, rate resistance ciprofloxacin, levofloxacin, amikacin, azithromycin 65, 75, 45 95% respectively. at 30 µg/ml reduced biomass 11 isolates; however stimulated 9 isolates. combination with imipenem 10 different exhibiting synergistic or antagonistic relationships.Conclusion: has increasingly developed many agents but less frequently been reported. However, could enhance production isolate- dependent manner because they may possibly utilize nanoparticle as an source, important element production. exact mechanism these however, remains be elucidated.