Negativization rates of IgE radioimmunoassay and basophil activation test in immediate reactions to penicillins.

摘要: Background:  Skin test sensitivity in patients with immediate allergy to penicillins tends decrease over time, but no information is available concerning vitro tests. We analysed the negativization rates of two methods that determine specific immunoglobulin E (IgE) antibodies, basophil activation using flow cytometry (BAT) and radioallergosorbent (RAST), allergic reactions penicillins. Methods:  Forty-one amoxicillin were followed up a 4-year period. BAT RAST performed at 6-month intervals. Patients randomized into groups: Group I, skin tests carried out regular intervals; II, made only beginning study. Results:  Differences observed between (P < 0.01), latter showing earlier negativization. Considering different haptens, significant differences for rate found (P < 0.05). Comparisons Groups I (n = 10) II (n = 31) showed tendency become negative later RAST. Conclusions:  Levels IgE antibodies tended time amoxicillin. Conversion took longer assay, although detected hapten. testing influenced contributing maintenance sensitivity. Because loss determination must be done as soon possible after reaction.