Basophil and eosinophil accumulation and mast cell degranulation in the nasal mucosa of patients with hay fever after local allergen provocation.

摘要: Abstract Background: Basophils and mast cells have certain similarities are believed to be important in upper lower respiratory allergy. Objective: We sought apply immunohistology investigate the distribution numbers of basophils nasal mucosa after allergen provocation. Methods: Allergen challenge with grass pollen was performed 9 patients seasonal allergic rhinitis out hay fever season. Nasal biopsy specimens were taken before approximately 1 hour, 24 hours, week intranasal determined relative their phenotypic characteristics by using mAbs specific for tryptase, chymase, IgE, eosinophils (BMK-13), a new mAb against (BB1) immunohistochemistry frozen sections. Results: In at baseline, practically no found epithelium. A significant increase epithelium lamina propria early phase as hour At hours provocation, basophil only. The proportion staining chymase decreased from median 38% (range, 0%-82%) 14% 0%-78%) within increased 1% 0%-86%) baseline 21% 3%-85%) One returned numbers. definite tissue eosinophilia observed Conclusion: Basophil subjects challenge, influx already apparent hour. Moreover, changes cell percentages (J Allergy Clin Immunol 2000;106:677-86.)