Crucial role of macrophages in matrix metalloproteinase-mediated cartilage destruction during experimental osteoarthritis: involvement of matrix metalloproteinase 3.

摘要: Objective To explore the involvement of synovial macrophages in early cartilage damage osteoarthritis (OA), and to identify role matrix metalloproteinase 3 (MMP-3) pathology late OA. Methods The MMP-mediated OA was studied by depleting prior elicitation a collagenase-induced instability model OA. The expression MMP synovium monitored using TaqMan analysis. In spontaneous induced OA, scored MMP-3–knockout mice control mice, histologic assessment VDIPEN staining. Results On day 14 following induction neoepitopes were detected from with mild experimental (mean ± SD positively stained surface area 20 3.2%). Remarkably, generation MMP-induced largely prevented 5 1%; P< 0.001), indicating an important for occurrence damage. We observed strong decrease MMP-3 MMP-9 but not tissue macrophage-depleted joints. Among 2-year-old OA–like changes lining layer significantly decreased compared mice. Even more striking 67% reduction severe addition, decreased, reduced breakdown. Conclusion The results this study prove that is involved murine Synovial are crucial activity appear mediate production rather than cartilage.