A comparative study of enzyme immobilization strategies for multi-walled carbon nanotube glucose biosensors

摘要: This work addresses the comparison of different strategies for improving biosensor performance using nanomaterials. Glucose biosensors based on commonly applied enzyme immobilization approaches, including sol‐gel encapsulation approaches and glutaraldehyde cross-linking strategies, were studied in presence absence multi-walled carbon nanotubes (MWNTs). Although direct design parameters such as linear range sensitivity is intuitive, this alone not an accurate indicator efficacy, due to wide electrodes nanomaterials available use current designs. We proposed a comparative protocol which considers both active area transduction following nanomaterial deposition sensitivity. Based protocol, when no involved, TEOS/GOx exhibited highest followed by BSA/GA/GOx TMOS/GOx biosensors. A novel containing carboxylated MWNTs modified with glucose oxidase overlying TMOS layer demonstrated optimum efficacy terms enhanced density (18.3 ± 0.5 μ Am M −1 cm −2 ), (0.0037‐12 mM), detection limit (3.7 μM), coefficient variation (2%), response time (less than 8 s), stability/selectivity/reproducibility. H2O2 tests that most possible reason enhancement was increased loading. excellent platform versatile biosensing applications.