Smooth Muscle Cell α2δ-1 Subunits Are Essential for Vasoregulation by CaV1.2 Channels
作者： John P. Bannister , Adebowale Adebiyi , Guiling Zhao , Damodaran Narayanan , Candice M. Thomas
DOI: 10.1161/CIRCRESAHA.109.203620
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摘要: Rationale: Voltage-dependent L-type (CaV1.2) Ca2+ channels are a heteromeric complex formed from pore-forming α1 and auxiliary α2δ β subunits. CaV1.2 the principal influx pathway in arterial myocytes regulate multiple physiological functions, including contraction. The macromolecular composition of myocyte remains poorly understood, with no studies having examined molecular identity or functions Objective: We investigated functional significance subunits resistance-size (100 to 200 μm diameter) cerebral arteries. Methods Results: α2δ-1 was only isoform expressed artery myocytes. Pregabalin, an α2δ-1/-2 ligand, antibody, inhibited currents isolated Acute pregabalin application reversibly dilated pressurized Using novel surface biotinylation, data indicated that >95% were present ...
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