Insertion into the Mycobacterium smegmatis genome of the aph gene through lysogenization with the temperate mycobacteriophage Ms6.
作者： Elsa Anes , Isabel Portugal , J. Moniz-Pereira
DOI: 10.1016/0378-1097(92)90731-3
关键词:
摘要: A derivative of the temperate mycobacteriophage Ms6 containing aph gene from transposon Tn 5 was constructed. In transductants integrated in bacterial genome. The is stably maintained absence positive selection after more than 150 generations. results presented this report show that can be used as a vehicle for integration foreign DNA into Mycobacterium smegmatis
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