Increased Adipogenic and Decreased Chondrogenic Differentiation of Adipose Derived Stem Cells on Nanowire Surfaces.

摘要: Despite many advances in tissue engineering, there are still significant challenges associated with restructuring, repairing, or replacing damaged the body. Currently, a major obstacle has been trying to develop scaffold for cartilage engineering that provides correct mechanical properties endure loads articular joints as well promote cell-scaffold interactions aid extracellular matrix deposition. In addition, adipogenic is widely growing due an increased need more innovative reconstructive therapies following adipose traumas and cosmetic surgeries. Recently, lipoaspirate identified viable alternative source mesenchymal stem cells because it contains supportive stroma can easily be isolated. Adipose derived (ADSCs) differentiate into variety of mesodermal lineages including chondrogenic phenotypes. Biodegradable polymeric scaffolds have shown promising used evaluate compatibility, viability, bioactivity these materials. Polycaprolactone bioresorbable polymer, which biomedical applications. The fundamental concept behind successful synthetic tissue-engineered progenitor cell migration, adhesion, proliferation, induce differentiation, synthesis, finally integration host tissue. this study, we investigated differentiation ADSCs on nanowire surfaces. A solvent-free gravimetric template technique was fabricate polycaprolactone nanowires results indicated during growth period i.e., initial 7 days culture, surfaces (NW) supported adhesion proliferation had elongated morphologies. However, without non-elongated Further, immunofluorescence imaging marker proteins showed did not appear support whereas ADSCs.