Prominence of IgG4 in the IgG antibody response to human filariasis.

摘要: The four subclasses of IgG are distinct in structure, function, and degree participation the response to complex antigens. Because these differences could have important pathogenetic significance, we analyzed total filaria antigen-specific each subclass 31 patients with different clinical manifestations Bancroftian filariasis. Subclass-specific, affinity-purified polyclonal antibodies were prepared from antisera raised sheep immunized purified myeloma proteins. These radiolabeled (125I) used detect IgG1, IgG2, IgG3, IgG4 solid phase radioimmunoassays (SPRIA). SPRIA was Brugia malayi adult antigen (BmA) bound Sepharose 4B, whereas measurement levels serum goat anti-human matrix. Quantification by reference WHO 67/97 standard, specific antibody development standards high titered sera. Although there modest increases IgG1 IgG2 filariasis compared normals, most striking finding extreme elevation both total, particularly, IgG4. elevations seen for essentially all patients, but relative proportion accounted particularly marked (up 95%) either microfilaremia or tropical pulmonary eosinophilia syndrome. meaning this special prominence filarial infection is not yet clear, question whether play a role immediate hypersensitivity reactions as reagins blocking being investigated its potential significance.