Stabilization of bovine insulin against agitation-induced aggregation using RNA aptamers.

摘要: Abstract Stabilization of monomeric insulin is a primary requirement for preserving the efficacy final formulation. Degraded and/or aggregated protein as well presence any conventional excipients can result in immunogenic or anaphylactic reactions, and reduced bioavailability drug. The aim this work was to select novel RNA-based stabilizers bovine which would recognize bind help retain its bioactivity. RNA aptamers were selected by an vitro selection method. They screened their ability inhibit fibrillation using agitation stress condition. activity determined phosphorylation downstream proteins cell. In vivo bioactivity diabetic rat model. aptamers, bound with very low dissociation constants high specificity, selected. These sequences aligned consensus regions found. had no effect on signalling cascade initiated insulin. insulin, measured lower plasma glucose level model, also remained unchanged. are class have disrupt protein–protein interactions hence aggregation. Their non-toxic non-immunogenic nature makes such formulations safe use.