A microfluidic-based hybrid SPR/molecular imaging biosensor for the multiplexed detection of foodborne pathogens

摘要: ABSTRACT It is important to screen our food supply for pathogen contaminations. Current methods bacterial contamination involve using costly reagents such as antibodies or PCR time-costly growth in cultures. There need portable, real-time, multiple x detection technology that can predict the safety of where it produced distributed. Surface plasmon resonance (SPR) imaging a sensitive, la bel-free method detect binding an analyte surface due changes refractive index th at occur upon binding. be used label–free presence potential pathogens. Simulta neous fluorescence molecular on other side biochip ascertain status functional state which may affect its danger humans animals. We are designing and testing hybrid microfluidic biochips pathogens combination SPRI imaging. The device consists array gold spots, each functionalized with peptide targeting specific pathogen. This biosensor arra y enclosed by PDMS flow chamber delivers magnetically concentrated sample tested. An SPR image taken from bottom biochip. Image analysis quantify amount (both live dead ) bound spot. Since very transmissive visible light, epi-fluorescence top Fluorescence determines live:dead ratio inexpensive SYTO 9®-Propidium Iodide assay. volume analyze small, so possible pre-concentrated immunomagnetic separation. Functionaliz ed magnetic particles present sample, magnet separate them bulk fluid. KEY TERMS: foodborne pathog ens; microfluidic; imaging; resonance; E. coli O157:H7