Cross-linked gelatin microsphere-based scaffolds as a delivery vehicle of MC3T3-E1 cells: in vitro and in vivo evaluation.

摘要: Abstract Scaffolding plays a crucial role in bone tissue engineering by not only providing interfaces for cell adhesion, proliferation, and differentiation but also guiding neotissue formation. For this purpose, microspheres (MSs) are being increasingly used alone or combination with other scaffolds. However, few researchers have MSs to prepare 3D scaffolds culture delivered cells. In study, we developed covalent cross-linked gelatin (ccG-MSs) (average diameter = 100–300 μm) load mouse osteoblast MC3T3-E1 cells, which exhibit attachment spreading on surfaces of ccG-MSs after co-culture. Significantly, the can be integrated into macroscopic construct cells 5 days cultivation. The within constructs show higher viability proliferation activity than those micro-cavitary gel (MCG) constructs. Calcium deposition, alkaline phosphatase as well osteocalcin secretion both MCG been evaluated vitro vivo, respectively. Compared scaffolds, ccG-MS-based provide better cellular microenvironments osteogenic differentiation. Our findings will lay foundation understanding behaviors MS-based help designing