Analytical techniques to study microbial biofilm on abiotic surfaces: pros and cons of the main techniques currently in use.

摘要: Biofilm is a bacterial lifestyle widespread in microbial world and represents concern health care. Despite the great life expectancy related to advanced care, increasing numbers of biofilm-mediated infections remain significant public challenge. Moreover, problem becomes much more severe when biofilm colonizes medical devices biomaterials. The risk due biofilm-related that requires full attention. However, complexity makes difficult its exhaustive analysis. Although major challenge both microbiological hygiene areas, at now methods aimed analyse formation development are not standardized yet. Different have been employed qualitatively quantitatively evaluate each which useful estimate peculiar aspect lifestyle. In present review, fifteen assays for qualitative quantitative evaluation colonizing abiotic substrates, such as devices, prosthesis or surfaces food production together with advantages limitations method were described compared. Some suited quantify matrix while others capable living dead cells exclusively viable biofilm. particular, colorimetric (crystal violet; 1,9-dimethyl methylen blue fluorescein-di-acetate methods) (LIVE/DEAD BacLight, BioTimer Assay, resazurin, tetrazolium hydroxide salt genetic population (PCR FISH) reported. section dedicated examine performances microscopic techniques study biofilms (mass spectrometry; confocal laser scanning microscopy; Raman spectroscopy electron microscopy). Because complexity, an combination different experimental approaches biochemical, physical ones.