Chapter 7: Total internal reflection fluorescence microscopy.

摘要: Total internal reflection fluorescence microscopy (TIRFM), also known as evanescent wave microscopy, is used in a wide range of applications, particularly to view single molecules attached planar surfaces and study the position dynamics organelles living culture cells near contact regions with glass coverslip. TIRFM selectively illuminates fluorophores only very thin (less than 100 nm deep) layer substrate, thereby avoiding excitation outside this subresolution optical section. This chapter reviews history, current applications cell biology biochemistry, basic theory, combinations numerous other spectroscopic approaches, setup methods, both commercial custom.