p53 Status Does Not Affect Sensitivity of Human Ovarian Cancer Cell Lines to Paclitaxel

摘要: Nine human ovarian cancer cell lines that express wild-type (wt) or mutated p53 were used to evaluate the cytotoxicity induced by paclitaxel. The IC50 calculated in five p53-expressing was not different from four wt lines. introduction of p53, using a temperature-sensitive mutant murine under control tetracycline-dependent promoter, did change paclitaxel as compared mock-transfected cells. By for each line IC50, we found these concentrations sufficient induce an increase levels all cells, whereas unaffected. This led mRNA and protein downstream genes (WAF1, GADD45, bax). In none examined able apoptosis, evaluated terminal deoxynucleotidyl transferase-mediated nick end labeling staining filter binding assay at closed IC50. increasing concentration assay, could see fragmentation DNA We conclude presence is determinant Differences activation be observed versus but this does account either differential induction apoptosis