Changes in Periodontal Health Status Are Associated with Bacterial Community Shifts as Assessed by Quantitative 16S Cloning and Sequencing

摘要: The gingival sulcus contains a complex ecosystem that includes many uncultivated bacteria. Understanding the dynamics of this in transitions between health and disease is important advancing our understanding bacterial etiology periodontitis. objective longitudinal study was to examine stability colonization crevice explore relationship shifts microbial composition changes periodontal status using comprehensive, quantitative, culture-independent approach. Subgingival plaque samples data were collected from 24 subjects over 2 years. Baseline 2-year analyzed quantitative ribosomal 16S cloning sequencing. Ten remained periodontally healthy years, seven worsened, showed clinical improvement. Bacterial greatest among healthy, clinically stable lowest for whose worsened (P = 0.01). Higher numbers species lost or gained also observed changed 0.009). This provides evidence change accompanied by within community. Based on these data, measures may be useful diagnosis prognosis. Regarding individual species, increases levels phylotype Veillonella sp. oral clone X042, gram-negative bacterium most common member subgingival community, associated with 0.04), suggesting an beneficial species. Filifactor alocis, gram-positive anaerobe, found at higher