The biological effects of titanium corrosion products on gingival epithelium

摘要: Implanted titanium (Ti) devices such as dental implants have been shown to produce metallic species within adjacent tissues. The effect of the presence these oral epithelial tissues is currently not well characterised or known. This thesis investigates effects TiO\(_2\) nanoparticles (TiO\(_2\) NPs) at a range concentrations on cells in context cell viability, cellular functions and interactions via variety methods. A co-culture model was established, difficulties using nano-scale insoluble stimulus were explored, high content screening techniques be potentially more appropriate methods than conventional assays this context. Interactions between TiO2 NPs imaged investigated imaging techniques. Oral take up vacuole type structures. Cell viability appeared affected lower concentrations. Gene expression changes response absence pathogenic bacteria investigated. Cytokines important cell-cell signalling bind NPs, therefore creating potential for modify immune responses implanted Ti devices.