Eukaryotic microorganisms in cold environments: examples from Pyrenean glaciers.

摘要: Little is known about the viability of eukaryotic microorganisms preserved in icy regions. Here we report on diversity microbial eukaryotes ice samples derived from four Pyrenean glaciers. The species composition communities these glaciers unknown mostly because presence a multi-year cap, and it not clear whether they harbor same populations. recent deglaciation areas allowing an easy access to glacial layers that correspond “Little Ice Age” although some isolated deposits are attributed previous cycles. In this study, use molecular 18S rRNA-based approaches characterize populations associated with Firstly, performed chemical microscopical characterization samples. Secondly, analyses revealed interesting protist genetic order understand resident were examined by amplifying community DNA constructing clone libraries rRNA primers. After removal potential chimeric sequences derreplication identical sequences, phylogenetic analysis demonstrated several different protists could be identified. Protist was more phylum rich Aneto Monte Perdido dominant taxonomic groups across all (> 1 % sequences) Viridiplantae Rhizaria. Significant variations relative abundances phyla between higher lower observed. At genus level, significant differences also recorded for genera Chloromonas, Raphidonema , Heteromita Koliella Bodomorpha. addition, structure showed at levels correlated altitude area pH.