Functional Genomics in the Mouse: Phenotype-Based Mutagenesis Screens

摘要: Significant progress has been made in sequencing the genomes of several model organisms, and efforts are now underway to complete human genome. In parallel with this effort, new approaches being developed for elucidation functional content The mouse will have an important role phase genome project as a system. review we discuss compare classical genetic gene function-phenotype-based mutagenesis screens aimed at establishment large collection single mutations affecting wide range phenotypic traits mouse. Whereas scale genome-wide that directed identification all loci contributing specific phenotype may be impractical, region-specific saturation provide within delimited chromosomal region feasible alternative. Region-specific can performed only two generations by combining high-efficiency chemical deletion complexes generated using embryonic stem (ES) cells. ability create analyze rapidly, well map novel chemically-induced these complexes, facilitate systematic analysis corresponding sequences humans. Furthermore, extent effort is still uncertain, underscore necessity direct those regions targets extensive screens.