Plasma biomarker screening for liver fibrosis with the N-terminal isotope tagging strategy

摘要: A non-invasive diagnostic approach is crucial for the evaluation of severity liver disease, treatment decisions, and assessing drug efficacy. This study evaluated plasma proteomic profiling via an N-terminal isotope tagging strategy coupled with liquid chromatography/Fourier transform ion cyclotron resonance mass spectrometry measurement to detect fibrosis staging. Pooled from different stages, which were assessed in advance by current gold-standard biopsy, was quantitatively analyzed. total 72 proteins found be dysregulated during fibrogenesis process, this finding constituted a valuable candidate biomarker bank follow-up analysis. Validation results fibronectin Western blotting reconfirmed mass-based data. Ingenuity Pathways Analysis showed four types metabolic networks functional effect disease chronic hepatitis B patients. Consequently, quantitative proteomics acetyl labeling technique provides effective useful tool screening biomarkers fibrosis. We monitored process CHB discovered many new diagnosis also partly identified mechanism involved disease. These provide clearer understanding pathophysiology will hopefully lead improvement clinical treatment.