Expression of fibronectin messenger RNA in hypertrophic and normal dermal tissues and in vitro regulation by interferon alfa-2b.

摘要: After severe thermal injury, hypertrophic scarring which is associated with accumulation of extracellular matrix proteins including fibronectin, frequently develops. We have recently demonstrated that interferon alfa-2b significantly reduces the level type 1 procollagen messenger RNA expressed by both and normal dermal fibroblasts. In this report, we provide evidence cytokine also decreases expression fibronectin in human scar Four fibroblast cell strains were established culture from four postburn tissues use fibroblasts same patients as controls. These cells then treated 2000 U/ml medium at various times. The results Northern analysis interferon-treated indicate reduced early 12 hours after treatment reached its lowest (24% relative to untreated fibroblasts) 96 hours. When was quantified densitometry for each individual paired strain, a differential response found among strains. decreased 17.2% 69% 47% 83.7% control values. Although decrease less pronounced than fibroblasts, reduction significant (6.39 +/- 0.71 versus 2.88 0.9, n = 4, p < 0.05) compared controls (5.47 0.89 3.64 0.99, assessed Student's t test. Rehybridization blot prepared complementary DNA tissue inhibitor metalloproteinase 2 gelatinase showed no changes abundance transcript. This result suggests selectively suppresses not due loading. A dot total extracted these carried out compare between dermis obtained patients. initially hybridized subsequently correct ratio or control, fourfold higher contrast, message cultured different study suggest developing injury an overexpression RNA, may be therapeutic value down-regulate