作者: Stefano Reale , Ettore Randi , Valentina Cumbo , Ignazio Sammarco , Floriana Bonanno
DOI: 10.1016/J.MAMBIO.2019.06.002
关键词:
摘要: Using next-generation sequencing, we obtained for the first time a complete mitochondrial DNA genome from museum specimen of extinct wolf (Canis lupus) population island Sicily (Italy). Phy¬logenetic analyses indicated that this genome, which was aligned with number historical and extant dog mitogenomes sampled worldwide, closely related to an Italian mtDNA (the observed proportion nucleotide sites at two sequences are different p= 0.0012), five seven times shorter than divergence among Sicilian any other known genomes (p range = 0.0050 - 0.0070). joined basal clade belonging haplogroup-2 ancient western European populations (Pilot et al., 2010). Bayesian calibration coalesced MRCA= 13.400 years (with 95% HPD 4000 -21.230 years). These findings suggest wolves probably colonized southern Italy towards end last Pleistocene glacial maximum when Strait Messina almost totally dry. Additional genomic data will further clarify origin dynamics before extinction in Sicily.