Non-housekeeping genes expressed in human trabecular meshwork cell cultures.

摘要: Purpose: To identify non-housekeeping genes definitively expressed in the human trabecular meshwork (TM). Methods: Microarray gene expression data on TM cultured cells from four studies were compared. Genes that queried at least three and assessed to be considered of TM. Housekeeping removed this set genes. The profile was analyzed for pathway enrichment microRNA targeting, using bioinformatics tools. results compared with previous non-array based studies. Results: Nine hundred sixty-two identified as Analysis these by Kyoto Encyclopedia Genomes led identification two enriched biologic pathways achieved a highly significant Bonferroni p-value (p≤0.01): focal adhesion extracellular matrix (ECM)-receptor interaction. Many previously implicated TM-related functions TM-associated disease glaucoma; however, some are novel. MicroRNAs known predicted target Ten here, ALDH1A1 (aldehyde dehydrogenase 1 family, member A1), CDH11 (cadherin 11, type 2, OBcadherin), CXCR7 (chemokine (C-X-C motif) receptor 7), CHI3L1 (chitinase 3-like 1), FGF2 (fibroblast growth factor 2), GNG11 (guanine nucleotide binding protein [G protein], gamma 11), IGFBP5 (insulin-like 5), PTPRM (protein tyrosine phosphatase, type, M), RGS5 (regulator G-protein signaling TUSC3 (tumor suppressor candidate 3), also reported earlier non-microarray Conclusions: A transcriptome consisting 962 identified. Multiple microRNAs proposed further study better understanding physiology.