Correction for incomplete labeling in the measurement of distance distributions by frequency-domain fluorometry
作者： Joseph R. Lakowicz , Ignacy Gryczyński , Wieslaw Wiczk , Józef Kuśba , Michael L. Johnson
DOI: 10.1016/0003-2697(91)90324-M
关键词:
摘要: Measurements of time-resolved fluorescence are now being used to recover conformational distributions biological macromolecules. The data the donor easily corrupted by incomplete labeling macromolecules acceptor. In present paper we describe a general procedure correct for acceptor in determination distance from frequency-domain measurements decay kinetics. method can also be determine extent labeling. Simulated were effect on resolution distribution and recovered if one fails account expressions implemented algorithm verified using known mixtures donor-control donor-acceptor pair molecules, which simulated presence population lacking Finally, between two labeled sites myosin S1 (Cys-697 Cys-707) where it was not possible obtain complete site.
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