T cell recognition of Mls. T cell clones demonstrate polymorphism between Mlsa, Mlsc, and Mlsd.

摘要: The determinants encoded by the minor lymphocyte stimulating locus (Mls) are defined as that induce strong T cell proliferative responses in primary mixed reactions. Although Mls was originally described having four alleles, a, b, c, and d, a number of recent observations have led several investigators to challenge idea is truly polymorphic system. To better define this system recognized at high frequency cells, present studies were undertaken evaluate polymorphism products. In study, vitro Mlsa- Mlsc-specific clones employed analyze identification Mlsc-reactive established pattern stimulators derived from congenic strains, recombinant inbred backcross mice. unprimed cells gave concordant confirmed Mlsa or Mlsc specificity cloned populations. With use these two sets Mls-specific clones, existence absence Mls-encoded gene products examined. It found Mlsa-specific responded but not stimulators, whereas Mlsa. This reciprocal indicates currently therefore polymorphic. addition, it observed both stimulated Mlsd stimulators. particular, possibility alleles different loci strains those express considered.