作者: A. P. Dabkowska , D. J. Barlow , R. A. Campbell , A. V. Hughes , P. J. Quinn
DOI: 10.1021/BM300639N
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摘要: The interaction of DNA with monolayers the cationic lipid dimethyldioctadecylammonium bromide, with/without 50 mol % a neutral “helper” lipid, either dioleoylphosphatidylethanolamine or cholesterol, has been studied using specular neutron reflection, surface pressure–area isotherms, and Brewster angle microscopy. amount bound to head groups comprehensively quantified in range 8–39 vol% respect monolayer composition (monolayers composed bromide binding most containing least) pressure (DNA being greatest at highest pressures). Surprisingly, regardless these variables, thickness DNA-containing layer remained approximately constant between 18 25 A. This systematic study is first direct quantification two different helper-lipid-containing multicomponent monolayers, an importan...