Effect of Helper Lipids on the Interaction of DNA with Cationic Lipid Monolayers Studied by Specular Neutron Reflection

作者: A. P. Dabkowska , D. J. Barlow , R. A. Campbell , A. V. Hughes , P. J. Quinn

DOI: 10.1021/BM300639N

关键词:

摘要: The interaction of DNA with monolayers the cationic lipid dimethyldioctadecylammonium bromide, with/without 50 mol % a neutral “helper” lipid, either dioleoylphosphatidylethanolamine or cholesterol, has been studied using specular neutron reflection, surface pressure–area isotherms, and Brewster angle microscopy. amount bound to head groups comprehensively quantified in range 8–39 vol% respect monolayer composition (monolayers composed bromide binding most containing least) pressure (DNA being greatest at highest pressures). Surprisingly, regardless these variables, thickness DNA-containing layer remained approximately constant between 18 25 A. This systematic study is first direct quantification two different helper-lipid-containing multicomponent monolayers, an importan...

参考文章(47)
R. Philip, D. Liggitt, M. Philip, P. Dazin, R. Debs, In vivo gene delivery. Efficient transfection of T lymphocytes in adult mice. Journal of Biological Chemistry. ,vol. 268, pp. 16087- 16090 ,(1993) , 10.1016/S0021-9258(19)85388-7
M A Whitt, L Buonocore, J K Rose, A new cationic liposome reagent mediating nearly quantitative transfection of animal cells. BioTechniques. ,vol. 10, pp. 520- 525 ,(1991)
Paula C. A. Barreleiro, Gerd Olofsson, Paschalis Alexandridis, Interaction of DNA with Cationic Vesicles: A Calorimetric Study Journal of Physical Chemistry B. ,vol. 104, pp. 7795- 7802 ,(2000) , 10.1021/JP000636C
Alain Léonard, Céline Escrive, Michel Laguerre, Eva Pebay-Peyroula, Willfrid Néri, Tanja Pott, John Katsaras, Erick J. Dufourc, Location of Cholesterol in DMPC Membranes. A Comparative Study by Neutron Diffraction and Molecular Mechanics Simulation Langmuir. ,vol. 17, pp. 2019- 2030 ,(2001) , 10.1021/LA001382P
D. McLoughlin, R. Dias, B. Lindman, M. Cardenas, T. Nylander, K. Dawson, M. Miguel, D. Langevin, Surface complexation of DNA with insoluble monolayers. Influence of divalent counterions. Langmuir. ,vol. 21, pp. 1900- 1907 ,(2005) , 10.1021/LA047700S
P. L. Felgner, T. R. Gadek, M. Holm, R. Roman, H. W. Chan, M. Wenz, J. P. Northrop, G. M. Ringold, M. Danielsen, Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure Proceedings of the National Academy of Sciences of the United States of America. ,vol. 84, pp. 7413- 7417 ,(1987) , 10.1073/PNAS.84.21.7413
Kai K. Ewert, Heather M. Evans, Alexandra Zidovska, Nathan F. Bouxsein, Ayesha Ahmad, Cyrus R. Safinya, A columnar phase of dendritic lipid-based cationic liposome-DNA complexes for gene delivery: hexagonally ordered cylindrical micelles embedded in a DNA honeycomb lattice. Journal of the American Chemical Society. ,vol. 128, pp. 3998- 4006 ,(2006) , 10.1021/JA055907H
Vladimir L. Shapovalov, Matthias Dittrich, Oleg V. Konovalov, Gerald Brezesinski, Use of Total Reflection X-ray Fluorescence (TRXF) for the Quantification of DNA Binding to Lipid Monolayers at the Air—Water Interface Langmuir. ,vol. 26, pp. 14766- 14773 ,(2010) , 10.1021/LA102472U
Jonathan P. Talbot, David J. Barlow, M. Jayne Lawrence, Peter A. Timmins, Giovanna Fragneto, Interaction of cationic lipoplexes with floating bilayers at the solid-liquid interface. Langmuir. ,vol. 25, pp. 4168- 4180 ,(2009) , 10.1021/LA802844F
Aliaksandr Klebanau, Natallia Kliabanova, Francisco Ortega, Francisco Monroy, Ramon G Rubio, Victor Starov, Equilibrium behavior and dilational rheology of polyelectrolyte/insoluble surfactant adsorption films: didodecyldimethylammonium bromide and sodium poly(styrenesulfonate). Journal of Physical Chemistry B. ,vol. 109, pp. 18316- 18323 ,(2005) , 10.1021/JP051862V