Composites of malonic acid diamides and phospholipids – Structural parameters for optimal transfection efficiency in A549 cells
作者： Christopher Janich , Christian Wölk , Stephanie Taßler , Simon Drescher , Annette Meister
关键词: Miscibility 、 Transfection 、 Lipofection 、 Organic chemistry 、 Alkyl 、 Cationic polymerization 、 Lipofectamine 、 Malonic acid 、 Biophysics 、 Chemistry 、 A549 cell
摘要: The aggregation behavior of various zwitterionic helper phospholipids, such as DOPE, DOPC, and DPPC, in combination with two new cationic lipids, namely TH4 OH4 (second generation malonic acid diamides) different molar ratios was studied regard to their physical–chemical properties. Additionally, lipoplexes prepared from these lipid mixtures were characterized respect the transfection efficacy using an EGFP-assay. fluid DOPE have shown comparable efficiency Lipofectamine 2000®. Furthermore, this report demonstrates huge influence on efficiency. Thereby, alkyl chain fluidity, miscibility charge density important efficient gene transfer. Practical applications: Although lipofection is a topic therapy since 1989, finding effective system lipids still process trial error. There much unknown about lipoplex formation well release genetic cargo. Aim presented work find parameters which are connected transfer. This transfer.
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Rumiana Koynova, Boris Tenchov, Cationic Lipids: Molecular Structure/Transfection Activity Relationships and Interactions with Biomembranes Topics in Current Chemistry. ,vol. 296, pp. 51- 93 ,(2010) , 10.1007/128_2010_67
Kai K. Ewert, Alexandra Zidovska, Ayesha Ahmad, Nathan F. Bouxsein, Heather M. Evans, Christopher S. McAllister, Charles E. Samuel, Cyrus R. Safinya, Cationic liposome-nucleic acid complexes for gene delivery and silencing: Pathways and mechanisms for plasmid DNA and siRNA Topics in Current Chemistry. ,vol. 296, pp. 191- 226 ,(2010) , 10.1007/128_2010_70
Paula C. A. Barreleiro, Gerd Olofsson, Paschalis Alexandridis, Interaction of DNA with Cationic Vesicles: A Calorimetric Study Journal of Physical Chemistry B. ,vol. 104, pp. 7795- 7802 ,(2000) , 10.1021/JP000636C
Paul E. Harper, David A. Mannock, Ruthven N.A.H. Lewis, Ronald N. McElhaney, Sol M. Gruner, X-Ray Diffraction Structures of Some Phosphatidylethanolamine Lamellar and Inverted Hexagonal Phases* Biophysical Journal. ,vol. 81, pp. 2693- 2706 ,(2001) , 10.1016/S0006-3495(01)75912-7
Baichao Ma, Shubiao Zhang, Huiming Jiang, Budiao Zhao, Hongtao Lv, Lipoplex morphologies and their influences on transfection efficiency in gene delivery Journal of Controlled Release. ,vol. 123, pp. 184- 194 ,(2007) , 10.1016/J.JCONREL.2007.08.022
P.R Cullis, P.W.M Van Dijck, B De Kruijff, J De Gier, Effects of cholesterol on the properties of equimolar mixtures of synthetic phosphatidylethanolamine and phosphatidylcholine. A 31P NMR and differential scanning calorimetry study Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 513, pp. 21- 30 ,(1978) , 10.1016/0005-2736(78)90108-6
Timothy P. O'Connor, Ronald G. Crystal, Genetic medicines: treatment strategies for hereditary disorders Nature Reviews Genetics. ,vol. 7, pp. 261- 276 ,(2006) , 10.1038/NRG1829
Derek Marsh, Lateral pressure in membranes Biochimica et Biophysica Acta. ,vol. 1286, pp. 183- 223 ,(1996) , 10.1016/S0304-4157(96)00009-3
Alfred Blume, Hansjörg Eibl, The influence of charge on bilayer membranes calorimetric investigations of phosphatidic acid bilayers Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 558, pp. 13- 21 ,(1979) , 10.1016/0005-2736(79)90311-0
Bjoern A. Bergenstaahl, Per. Stenius, Phase diagrams of dioleoylphosphatidylcholine with formamide, methylformamide and dimethylformamide The Journal of Physical Chemistry. ,vol. 91, pp. 5944- 5948 ,(1987) , 10.1021/J100307A026