Integrated Analysis of Intracellular Dynamics of MenaINV Cancer Cells in a 3D Matrix

摘要: The intracellular environment is composed of a filamentous network that exhibits dynamic turnover cytoskeletal components and internal force generation from molecular motors. Particle tracking microrheology enables means to probe the mechanics dynamics. Here, we develop an analytical model capture basic features active mechanical environment, including both thermal motor-driven effects, show consistency with diverse range experimental data. We further perform experiments, integrated Brownian dynamics simulations of the cytoskeleton, on metastatic breast cancer cells embedded in three-dimensional collagen matrix without presence epidermal growth factor response physiologically mimicking scenario. Our results demonstrate EGF stimulation can alter stiffness power output fluctuations overexpressing invasive isoform actin-associated protein Mena.