Evidence for cooperation between cells during sporulation of the yeast Saccharomyces cerevisiae.

摘要: Abstract Diploid Saccharomyces cerevisiae cells heterozygous for the mating type locus (MATa/MAT alpha) undergo meiosis and sporulation when starved nitrogen in presence of a poor carbon source such as potassium acetate. Diploid yeast adenine auxotrophs sporulated well at high cell density (10(7) per ml) under these conditions but failed to differentiate low (10(5) ml). The conditional sporulation-deficient phenotype could be complemented by wild-type cells, medium from cultures that sporulate density, or exogenously added (or hypoxanthine with some mutants). Adenine addition guanine, adenosine, numerous nucleotides were secreted into medium, each its unique temporal pattern, sporulating auxotrophic prototrophic strains. major compounds was degradation RNA. data indicated differentiating cooperate during maintaining sufficiently concentrations extracellular purines which are absolutely required auxotrophs. Yeast prototrophs, also less efficiently (10(3) ml), reutilized preference de novo-made purine whose synthesis fact inhibited density. enhanced prototrophs behavior bearing additional mutations salvage pathway genes (ade apt1, ade aah1 hpt1) supports model secretion products, uptake, reutilization products is signal between synchronizing process.