Arabinogalactan isolated from cowshed dust extract protects mice from allergic airway inflammation and sensitization

摘要: Background Extract from cowshed dust (CDE) is a source of immunomodulating substances. We have previously shown that such substances protect experimental allergic disorders in mouse model asthma. Objective The objective this study was to identify immunomodulatory molecules extracts an allergy protective farming environment. Methods Polysaccharides were isolated CDE and plants by chromatography precipitation with specific reagents. then characterized nuclear magnetic resonance spectroscopy. Subsequently, the allergy-protective potential polysaccharides tested Results authors demonstrate plant arabinogalactans are contained high concentrations. arabinogalactan fodder, particular prevalent grass species known as Alopecurus pratensis. Treatment murine dendritic cells resulted autocrine IL-10 production. Interestingly, these not able induce immune response. Furthermore, intranasal application protected mice developing atopic sensitization, airway inflammation hyperreactivity This effect for because control experiments gum arabic larch revealed do show properties. likely structural differences we spectroscopy although they predominantly composed arabinose galactose, differ structure. Conclusions conclude important contribute protection inflammation, hyperresponsiveness, sensitization