Ekstrak Metanol Daun Kelor Menurunkan Ekspresi BCL-2, TRAIL-R1, dan Kadar Caspase-3 Jaringan Kolon Tikus yang Diinduksi DMBA Methanol Extract of Moringa oleifera Decrease BCL-2 Expression, TRAIL-R1 and Caspase-3 Level of Rat Colon Tissue Induced DMBA

摘要: Methanol extract of Moringa oleifera leaf functioned as antioxidant, antiproliferation, or antitumor. This study purposes to confirm the effect methanol leaf, specifically NTT variety, protein apoptosis expression (TRAIL R1, Bcl-2, dan caspase-3) on wistar rat colon tissue induced by DMBA. Experimental research using post test group design 30 selected complete randomly. All rats homogenously conditioned and divided into 5 based var dose DMBA, given orally observed for 60 days. TRAIL-R1 bcl2 examination immunohistochemistry (Anti human/mouse/rat bcl-2 purified mouse monoclonal IgG2 Anti-m TRAIL R1 Rat IgG2A, R&D system), while Caspase-3 ELISA method (human/mouse caspase-3 Duoset IC® System). Data analysis was ANOVA correlation. Result showed that NTT, had lower 20 & 40 mg/kg body weight, but not in 80 weight. Those three doses also, gave Bcl-2 then positive control. There's no significant correlation between average among treatment (F=0,375, p=0,824). It can be concluded decreases weight all has