Human topoisomerase I promotes HIV-1 proviral DNA synthesis: implications for the species specificity and cellular tropism of HIV-1 infection.

摘要: Although HIV type 1 (HIV-1) cannot efficiently replicate in simian cells, the mechanism(s) involved restriction of virus tropism remain unclear. To investigate this, we have focused on identification human cellular factors that can influence infectivity HIV-1 derived from African green monkey producer cells. Whereas such cells was only 10–15% cell-derived virus, expression topoisomerase I resulted a 5-fold increase progeny virions. Replacement glutamate-236 and asparagine-237 with corresponding residues (aspartate serine, respectively) enzyme abolished this enhancement infectivity. This positive effect seemed to result promotion cDNA synthesis. Thus, plays an important role replication infectivity, differences species specificity infection at least part be attributed activities.